Alternative Views of Origin Of Life Essay Sample
•Discuss the ways in which developments in scientific cognition may conflict with the thoughts about the beginnings of life developed by different civilizations: Different civilizations and spiritual in the universe have their ain thoughts about life. it possibly rather different to the scientific evidenceIn scriptural creationism different beings were made for specialized environments at the same clip. the beings that were created have non changed nor are they related.
Science: Development ; all beings are invariably altering. non created. but evolved. Alternative Views of Origin Of Life Essay Sample.
Christians: They believe in Biblical creationism ; all organisms created as they are by God. no alteration over timeChinese: Believe the first being was P’an Ku. who evolved in a elephantine cosmic egg. All elements of the existence were in the egg. all assorted. In the egg. he separated the antonyms. so 18. 000 old ages subsequently the egg hatched. and P’an Ku died from the attempt of creative activity.
Aboriginals: Dreamtime ; great supernatural existences existed in the dreamtime and created the Earth and everything in itGreek: Aristotle’s thoughts that the whole existence had a hierarchy and that it started from stones. up through workss and animate beings. to worlds. and eventually to God.
Romans: Lucretius. a Roman philosopher believed there was no God. because the existence was so imperfect. It was made of atoms all squashing together.
As you can see. science contradicts with the belief of many people.
Conflicts with different culturesDifferent civilizations and spiritual in the universe have their ain thoughts about life. it possibly rather different to the scientific grounds.
¬In scriptural creationism different beings were made for specialized environments at the same clip. the beings that were created have non changed nor are they related¬Australian Aborigines believe that hereditary existences created the universe a long clip ago in a period known as the dream clip. Alternative Views of Origin Of Life Essay Sample. The scientific theories in this country are different to what the natives believe and this can frequently do struggle.
This struggle does nevertheless provoke people to believe about what might hold truly happened and to oppugn beliefs and leads to the formation of new theories•Since the proposal of the theory of development. there has been conflict between the spiritual and scientific positions of the beginnings of life.
•Depending on your POV – development = godless philistinism against morality or enlightened humanitarianism agaust superstitious notion. Alternative Views of Origin Of Life Essay Sample.
ORDER A PLAGIARISM-FREE PAPER NOW
•Most scientists support development. some have combined the theory with spiritual beliefs theistic development.
The Evolution-Creation argument: Traditional version -•End of eighteenth century – people believed in creative activity.
•Carolus Linnaeus believed that species had ne’er changed since creative activity.
•French anatomist Cuvier used cognition of life craniates to assist retrace the visual aspect of the many dodos being discovered at the clip. He said that the dodos were the remains of workss and animate beings killed by calamities like the scriptural inundation.
•Resemblance between populating things resulted from design. The Creator” showed his accomplishment in changing the inside informations while retaining the necessities.
The great debate•Since Darwin’s The Origin of Species was published in 1859. there was immediate reaction from the church.
•Darwin was instantly condemned everyplace. but was defended by a few influential scientists ( Lyell and Huxley ) .
•There was a battle at Oxford in 1860 over development – Huxley VS Bishop of Oxford.
Biology 1 ; J. Gao ; Mcmillan Press ; 2004 ;
Understanding the origin of cellular life on Earth requires the discovery of plausible pathways for the transition from complex prebiotic chemistry to simple biology, defined as the emergence of chemical assemblies capable of Darwinian evolution. We have proposed that a simple primitive cell, or protocell, would consist of two key components: a protocell membrane that defines a spatially localized compartment, and an informational polymer that allows for the replication and inheritance of functional information. Recent studies of vesicles composed of fatty-acid membranes have shed considerable light on pathways for protocell growth and division, as well as means by which protocells could take up nutrients from their environment. Additional work with genetic polymers has provided insight into the potential for chemical genome replication and compatibility with membrane encapsulation. The integration of a dynamic fatty-acid compartment with robust, generalized genetic polymer replication would yield a laboratory model of a protocell with the potential for classical Darwinian biological evolution, and may help to evaluate potential pathways for the emergence of life on the early Earth. Here we discuss efforts to devise such an integrated protocell model.
The emergence of the first cells on the early Earth was the culmination of a long history of prior chemical and geophysical processes. Although recognizing the many gaps in our knowledge of prebiotic chemistry and the early planetary setting in which life emerged, we will assume for the purpose of this review that the requisite chemical building blocks were available, in appropriate environmental settings. This assumption allows us to focus on the various spontaneous and catalyzed assembly processes that could have led to the formation of primitive membranes and early genetic polymers, their coassembly into membrane-encapsulated nucleic acids, and the chemical and physical processes that allowed for their replication. Alternative Views of Origin Of Life Essay Sample. We will discuss recent progress toward the construction of laboratory models of a protocell (Fig. 1), evaluate the remaining steps that must be achieved before a complete protocell model can be constructed, and consider the prospects for the observation of spontaneous Darwinian evolution in laboratory protocells. Although such laboratory studies may not reflect the specific pathways that led to the origin of life on Earth, they are proving to be invaluable in uncovering surprising and unanticipated physical processes that help us to reconstruct plausible pathways and scenarios for the origin of life.
A simple protocell model based on a replicating vesicle for compartmentalization, and a replicating genome to encode heritable information. A complex environment provides lipids, nucleotides capable of equilibrating across the membrane bilayer, and sources of energy (left), which leads to subsequent replication of the genetic material and growth of the protocell (middle), and finally protocellular division through physical and chemical processes (right). (Reproduced from Mansy et al. 2008 and reprinted with permission from Nature Publishing ©2008.)
The term protocell has been used loosely to refer to primitive cells or to the first cells. Here we will use the term protocell to refer specifically to cell-like structures that are spatially delimited by a growing membrane boundary, and that contain replicating genetic information. A protocell differs from a true cell in that the evolution of genomically encoded advantageous functions has not yet occurred. With a genetic material such as RNA (or perhaps one of many other heteropolymers that could provide both heredity and function) and an appropriate environment, the continued replication of a population of protocells will lead inevitably to the spontaneous emergence of new coded functions by the classical mechanism of evolution through variation and natural selection. Once such genomically encoded and therefore heritable functions have evolved, we would consider the system to be a complete, living biological cell, albeit one much simpler than any modern cell (Szostak et al. 2001).
BACKGROUND
Membranes as compartment boundaries
All biological cells are membrane-bound compartments. The cell membrane fulfills the essential function of creating an internal environment within which genetic materials can reside and metabolic activities can take place without being lost to the environment.Alternative Views of Origin Of Life Essay Sample. Modern cell membranes are composed of complex mixtures of amphiphilic molecules such as phospholipids, sterols, and many other lipids as well as diverse proteins that perform transport and enzymatic functions. Phospholipid membranes are stable under a wide range of temperature, pH, and salt concentration conditions. Such membranes are extremely good permeability barriers, so that modern cells have complete control over the uptake of nutrients and the export of wastes through the specialized channel, pump and pore proteins embedded in their membranes. A great deal of complex biochemical machinery is also required to mediate the growth and division of the cell membrane during the cell cycle. The question of how a structurally simple protocell could accomplish these essential membrane functions is a critical aspect of understanding the origin of cellular life.
Vesicles formed by fatty acids have long been studied as models of protocell membranes (Gebicki and Hicks 1973; Hargreaves and Deamer 1978; Walde et al. 1994a). Fatty acids are attractive as the fundamental building block of prebiotic membranes in that they are chemically simpler than phospholipids. Fatty acids with a saturated acyl chain are extremely stable compounds and therefore might have accumulated to significant levels, even given a relatively slow or episodic synthesis. Moreover, the condensation of fatty acids with glycerol to yield the corresponding glycerol esters provides a highly stabilizing membrane component (Monnard et al., 2002). Finally, phosphorylation and the addition of a second acyl chain yields phosphatidic acid, the simplest phospholipid, thus providing a conceptually simple pathway for the transition from primitive to more modern membranes. The prebiotic chemistry leading to the synthesis of fatty acids and other amphiphilic compounds is treated in more detail in Mansy (2010).
The best reason for considering fatty acids as fundamental to the nature of primitive cell membranes is not, however, their chemical simplicity. Rather, fatty-acid molecules in membranes have dynamic properties that are essential for both membrane growth and permeability. Because fatty acids are single chain amphiphiles with less hydrophobic surface area than phospholipids, they assemble into membranes only at much higher concentrations. This equilibrium property is mirrored in their kinetics: Fatty acids are not as firmly anchored within the membrane as phospholipids; they enter and leave the membrane on a time scale of seconds to minutes (Chen and Szostak 2004). Alternative Views of Origin Of Life Essay Sample.Fatty acids can also exchange between the two leaflets of a bilayer membrane on a subsecond time scale. Rapid flip-flop is essential for membrane growth when new amphiphilic molecules are supplied from the environment. New molecules enter the membrane primarily from the outside leaflet, and flip-flop allows the inner and outer leaflet areas to equilibrate, leading to uniform growth.
Considering that protocells on the early Earth did not, by definition, contain any complex biological machinery, they must have relied on the intrinsic permeability properties of their membranes. Membranes composed of fatty acids are in fact reasonably permeable to small polar molecules and even to charged species such as ions and nucleotides (Mansy et al. 2008). This appears to be largely a result of the ability of fatty acids to form transient defect structures and/or transient complexes with charged solutes, which facilitate transport across the membrane. The subject of the permeability of fatty-acid based membranes is dealt with in greater detail by Mansy (2010).
Prebiotic vesicles were almost certainly composed of complex mixtures of amphiphiles. Amphiphilic molecules isolated from meteorites (Deamer 1985; Deamer and Pashley 1989) as well as those synthesized under simulated prebiotic conditions (McCollum et al. 1999; Dworkin et al. 2001; Rushdi and Simoneit 2001) are highly heterogeneous, both in terms of acyl chain length and head group chemistry. Membranes composed of mixtures of amphiphiles often have superior properties to those composed of single pure species. For example, mixtures of fatty acids together with the corresponding alcohols and/or glycerol esters generate vesicles that are stable over a wider range of pH and ionic conditions (Monnard et al. 2002), and are more permeable to nutrient molecules including ions, sugars and nucleotides (Chen et al. 2004; Sacerdote and Szostak 2005; Mansy et al. 2008). This is in striking contrast to the apparent requirement for homogeneity in the nucleic acids, where even low levels of modified nucleotides can be destabilizing or can block replication. Alternative Views of Origin Of Life Essay Sample.
RECENT RESULTS
Pathways for Vesicle Growth
Fatty-acid vesicle growth has been shown to occur through at least two distinct pathways: growth through the incorporation of fatty acids from added micelles, and growth through fatty-acid exchange between vesicles. The growth of membrane vesicles from micelles has been observed following the addition of micelles or fatty-acid precursors to pre-formed vesicles (Walde et al. 1994a, 1994b; Berclaz et al. 2001). When initially alkaline fatty-acid micelles are mixed with a buffered solution at a lower pH, the micelles become thermodynamically unstable. As a consequence, the fatty-acid molecules can either be incorporated into pre-existing membranes, leading to growth (Berclaz et al. 2001), or can self-assemble into new vesicles (Blochliger et al. 1998; Luisi et al. 2004). These pioneering studies were done by cryo-TEM, which does not allow growth to be followed in real time, and by light scattering, which is difficult to interpret in the case of samples with heterogeneous size distributions. We therefore adapted a fluorescence assay based on FRET (Förster resonance energy transfer) to measure changes in membrane area in real time. This assay is based on the distance dependence of energy transfer between donor and acceptor fluorescent dyes; thus when a membrane grows in area by incorporating additional fatty-acid molecules, the dyes are diluted and the efficiency of FRET decreases. Studies on small (typically 100 nm in diameter) unilamellar fatty-acid vesicles using this assay showed that the slow addition of fatty-acid micelles led to vesicle growth with an efficiency of ∼90% (Hanczyc et al. 2003).
The real-time FRET assay allowed for a kinetic dissection of the growth process, revealing a surprisingly complex series of events after the rapid addition of micelles (Chen and Szostak 2004). Two major processes were observed. The first fast phase resulted in membrane area growth that was limited to ∼40% increase in area, independent of the amount of added micelles. A second much slower phase led to a further increase in membrane area that varied with the amount of added micelles. We interpreted the fast phase as reflecting the rapid assembly of a layer of adhering micelles around the pre-formed vesicles, with rapid monomer exchange resulting in the efficient incorporation of this material into the pre-formed membrane.Alternative Views of Origin Of Life Essay Sample. We interpreted the slow phase as the consequence of micelle–micelle interactions leading to the assembly of intermediate structures that could partition between two pathways—with some monomers dissociating and contributing to membrane growth and the remainder ultimately assembling into new membrane vesicles. Although these interpretations are consistent with our data, the experiments are rather indirect, and further exploration of the mechanism of membrane growth is certainly desirable.
A second, distinct pathway for vesicle growth involves fatty-acid exchange between vesicles. Under certain conditions this exchange can lead to growth of a subpopulation of vesicles at the expense of their surrounding neighbors. Within populations of osmotically relaxed vesicles, such exchange processes do not result in significant changes in size distribution with time. Similarly, a population of uniformly osmotically swollen vesicles does not change in size distribution, but such vesicles are in equilibrium with a lower solution concentration of fatty acids because the tension in the membrane of the swollen vesicles makes it more energetically favorable for fatty-acid molecules to reside in membrane. When osmotically swollen vesicles are mixed with osmotically relaxed (isotonic) vesicles, rapid fatty-acid exchange processes result in growth of the swollen vesicles and corresponding shrinkage of the relaxed vesicles (Chen et al. 2004). Because vesicles can be osmotically swollen as a result of the encapsulation of high concentrations of nucleic acids such as RNA, this process allows for the growth of vesicles containing genetic polymers at the expense of empty vesicles (or vesicles that contain less internal nucleic acid). Because faster replication would increase the internal nucleic acid concentration, this pathway of competitive vesicle growth provides the potential for a direct physical link between the rate of replication of an encapsulated genetic polymer and the rate of growth of the protocell as a whole.
Assuming that the division of osmotically swollen vesicles could occur either stochastically or at some threshold size, protocells that developed some heritable means of faster replication and growth would have a shorter cell cycle, on average, and would therefore gradually take over the population. This simple physical mechanism might therefore lead to the emergence of Darwinian evolution by competition at the cellular level. However, if replication is limited by the rapid reannealing of complementary strands (see later discussion), it may be difficult to reach osmotically significant concentrations. Alternative Views of Origin Of Life Essay Sample. Furthermore, osmotically swollen vesicles are intrinsically difficult to divide owing to the energetic cost of reducing the volume of a spherical vesicle to that of two daughter vesicles of the same total surface area. One possibility is that osmotically driven competitive growth might alternate with the faster membrane growth that follows micelle addition. If new fatty-acid material was only available sporadically, rapid membrane growth might follow an influx of fresh fatty acids, facilitating division (see following discussion).
All of the experiments discussed earlier were done with small unilamellar vesicles prepared by extrusion through 100 nm pores in filters. In contrast, fatty-acid vesicles that form spontaneously by rehydrating dry fatty-acid films tend to be several microns in diameter and multilamellar (Hargreaves and Deamer 1978; Hanczyc et al. 2003). Such large multilamellar fatty-acid vesicles are so heterogeneous that quantitative studies of growth and division are difficult. We have recently developed a simple procedure for the preparation of micron-sized, monodisperse (homogeneous in size) multilamellar vesicles by large-pore dialysis (Zhu and Szostak 2009b). The preparation of large monodisperse multilamellar vesicles has allowed us to directly observe an unusual mode of vesicle growth (Zhu and Szostak 2009a). We showed that feeding a micron-sized multilamellar fatty-acid vesicle with fatty-acid micelles results in the formation of a thin membranous protrusion which extends from the side of the initially spherical parental vesicle. Over time, this thin membrane tubule elongates and thickens, gradually incorporating more and more of the parental vesicle, until eventually the entire vesicle is transformed into a long, hollow threadlike vesicle (Fig. 2). This pathway occurs with vesicles ranging in size from 1 to at least 10 µm in diameter, composed of a variety of different fatty acids and related amphiphiles. Only multilamellar vesicles grow in this manner and only when vesicle volume increases slowly (relative to surface area growth) because of a relatively impermeable buffer solute. Confocal microscopy has provided insight into the mechanism of this mode of growth: The outermost membrane layer grows first, and because there is little volume between it and the next membrane layer, and that volume cannot increase on the same time scale, the extra membrane area is forced into the form of a thin tubule. Over time, this tubule grows, and as a result of poorly understood exchange processes, the entire original vesicle is ultimately transformed into a long thread-like hollow vesicle (Fig. 3). Alternative Views of Origin Of Life Essay Sample.
Vesicle shape transformations during growth. All vesicles are labeled with 2 mM encapsulated HPTS, a water-soluble fluorescent dye, in their internal aqueous space. (A) 10 min and (B) 30 min after the addition of five equivalents of oleate micelles to oleate vesicles (in 0.2 M Na-bicine, pH 8.5). Scale bar: 50 µm. (Reproduced from Zhu and Szostak 2009a and reprinted with permission from ACS Publications ©2009.)
Schematic diagram of coupled vesicle growth and division. (Reproduced from Zhu and Szostak 2009a and reprinted with permission from the Journal of the American Chemical Society ©2009.)
Pathways for Vesicle Division
Vesicle division by the extrusion of large vesicles through small pores is a way in which mechanical energy can be used to drive division (Hanczyc et al. 2003). Vesicle growth by micelle feeding followed by division by extrusion can be performed repetitively, resulting in cycles of growth and division in which both membrane material and vesicle contents are distributed to daughter vesicles in each cycle. However, division by extrusion results in the loss of 30%–40% of the encapsulated vesicle contents to the environment during each cycle (Hanczyc et al. 2003; Hanczyc and Szostak 2004). Most of this loss is a result of the unavoidable geometric constraint of dividing a spherical vesicle into two spherical (or subspherical) daughter vesicles with conservation of surface area; some additional loss may occur as a result of pressure-induced membrane rupture. Although extrusion is a useful laboratory model for vesicle division, an analogous extrusion process appears unlikely to occur in a prebiotic scenario on the early Earth because vesicle extrusion from the flow of suspended vesicles through a porous rock would require both the absence of any large pores or channels and a very high pressure gradient (Zhu and Szostak 2009a).
The above problems stimulated a search for a more realistic pathway for vesicle division. The possible spontaneous division of small unilamellar vesicles after micelle addition has been reported (Luisi et al. 2004; Luisi 2006), and electron microscopy has revealed structures that are possible intermediates in growth and division, notably pairs of vesicles joined by a shared wall (Stano et al. 2006). Alternative Views of Origin Of Life Essay Sample. However, the mechanism of the proposed division as well as the nature of the energetic driving force remain unclear. Additional studies are required to clearly distinguish between the vesicle-stimulated assembly of new vesicles, and the more biologically relevant processes of growth and division.
We have recently found that the growth of large multilamellar vesicles into long threadlike vesicles, described above, provides a pathway for coupled vesicle growth and division (Zhu and Szostak 2009a). The long threadlike vesicles are extremely fragile, and divide spontaneously into multiple daughter vesicles in response to modest shear forces. In an environment of gentle shear, growth and division become coupled processes because only the filamentous vesicles can divide (Fig. 3). If the initial parental vesicle contains encapsulated genetic polymers such as RNA, these molecules are distributed randomly to the daughter vesicles and are thus inherited. The robustness and simplicity of this pathway suggests that similar processes might have occurred under prebiotic conditions. The mechanistic details of this mode of division remain unclear. One possibility, supported by some microscopic observations, is that the long thin membrane tubules are subject to the “pearling instability” (Bar-Ziv and Moses 1994), and minimize their surface energy by spontaneously transforming from a cylindrical shape to a string of beads morphology. The very thin tether joining adjacent spherical beads may be a weak point that can be easily disrupted by shear forces.
RNA-Catalyzed RNA Replication on the Early Earth and the Modern Laboratory
A core assumption of the RNA world hypothesis is that the RNA genomes of primitive cells were replicated by a ribozyme RNA polymerase (Gilbert 1986). The idea of RNA-catalyzed RNA replication provides a solution to the apparent paradox of DNA replication catalyzed by proteins that are encoded by DNA. This simplification of early biochemistry gained instant plausibility from the discovery of catalytic RNAs almost 30 years ago (Kruger et al. 1982; Guerrier-Takada et al. 1983). Alternative Views of Origin Of Life Essay Sample. In the time after the discovery of the first ribozymes, the RNA World hypothesis has continued to gain support, most dramatically from the discovery that the ribosome is a ribozyme (Nissen et al. 2000), and that all proteins are assembled through the catalytic activity of the ribosomal RNA. Support has also come from the in vitro evolution of a wide range of new ribozymes, including bona fide RNA polymerases made of RNA (Johnston et al. 2001). On the other hand, no ribozyme polymerase yet comes close to being a self-replicating RNA, like the replicase envisaged as the core of the RNA World biochemistry.
Why has the in vitro evolution of an RNA replicase been so much more difficult than originally expected? It is clear that the problem is not with catalysis of the chemical step, even with catalytically demanding triphosphate substrates. Evolutionarily optimized versions of the Class I ligase carry out multiple-turnover ligation reactions at >1 s−1, with over 50,000 turnovers overnight (Ekland et al. 1995), and optimized versions of the smaller, simpler DSL ligase carry out sustained multiple turnover ligation reactions at rates >1/min (Voytek and Joyce 2007). These catalytic rates are more than sufficient to carry out the replication of a 100–200 nt ribozyme in minutes to hours, if these rates could be maintained in the context of a polymerase reaction using monomer substrates. However, even the best available polymerase ribozyme requires 1–2 days to copy 10–20 nucleotides of a template strand, apparently as a consequence of poor binding to both the ribonucleoside triphosphate (NTP) monomers and the primer-template substrate. The need to overcome the electrostatic repulsion between negatively charged NTP and RNA substrates and ribozyme is thought to contribute to the very high Mg++ requirement for the polymerase ribozyme (Glasner et al. 2000). Such high levels of Mg++ lead to hydrolytic degradation of the ribozyme, and are also not compatible with known fatty-acid based membranes because of crystallization of the fatty acid-magnesium salt. In addition, fatty-acid membranes are almost impermeable to NTPs (Mansy et al. 2008). Alternative Views of Origin Of Life Essay Sample.
The incompatibility between currently available ribozyme polymerases and fatty-acid based vesicles suggests either that early replicases were quite different, or that RNA replication in early cells proceeded in a very different manner. For example, less charged and more activated nucleotides might be easier for a ribozyme polymerase to bind, with little or no Mg++. Many potential leaving groups, such as imidazole, adenine or 1-Me-adenine have been examined in template-directed and nontemplated polymerization reactions, but have not yet been tested as substrates for ribozyme polymerases (Prabahar and Ferris 1997; Huang and Ferris 2006). The tethering of ribozyme and primer-template to hydrophobic aggregates has been examined as a way of increasing local substrate concentration (Müller and Bartel 2008). However, this approach did not lead to a dramatic improvement in the extent of template copying, apparently as a result of ribozyme inhibition at high effective RNA concentrations. It may be possible to evolve polymerases that operate well at high RNA concentrations, but membrane localization by chemical derivatization adds further complexity to a replication pathway because of the need for a specific catalyst for the derivatization step. Alternative means of facilitating the interaction of a ribozyme with a primer-template substrate, such as the presence of basic peptides or other cofactors, might overcome this problem. Finally it is noteworthy that very small self-aminoacylating ribozymes have been obtained using RNA libraries that have an unconstrained sequence at their 3′-end (Chumachenko et al. 2009). This strat egy may also be fruitful in selections for ribozyme polymerases.
ORDER A PLAGIARISM-FREE PAPER NOW
Given the above constraints and uncertainties, what can we say about the emergence of RNA-catalyzed RNA replication in the origin of life? It is still possible that under the proper conditions, and using the right substrates, a small simple ribozyme could effectively catalyze RNA replication. However, a replicase must do more than catalyze a simple phospho-transfer reaction. Binding in a nonsequence-specific manner to a primer-template complex, facilitating binding of the proper incoming monomer, catalyzing primer extension, and repeating this process until the end of the template (or set of templates for a multi-component replicase) is reached might require a complex replicase structure. Such a replicase would presumably be rare in collections of random RNA sequences.Alternative Views of Origin Of Life Essay Sample. If life required a very special sequence to get started, then the origin of life on earth could have been a low probability event and life on other earth-like planets might be very rare. If, on the other hand, RNA-catalyzed RNA replication could have emerged gradually in a series of simpler steps, it might have been easier and thus more likely for life to begin, and life elsewhere might be common. For this reason, we now turn to a consideration of nonenzymatic template-directed replication chemistry.
Chemical Template Replication Revisited
The nonenzymatic template-directed polymerization of activated ribonucleotides was studied in depth by Leslie Orgel, together with his students and colleagues, over a period of several decades (Orgel 2004). Here, the template itself acts as a catalyst by helping to align and orient the monomers so that they are pre-organized for polymerization. The main lesson from this work is that spontaneous chemical copying of RNA sequences is indeed possible, but is subject to several important constraints and limitations. The constraints make template-directed RNA copying incompatible with currently available membrane vesicle systems, and the limitations have, so far at least, made it impossible to obtain repeated cycles of RNA replication through chemical copying. Alternative Views of Origin Of Life Essay Sample.